In this way it also can be used to indirectly follow the reaction kinetics of any hydrogen peroxide-producing enzyme, or to simply quantify the amount of hydrogen peroxide in a sample. Its use allows the reaction kinetics of peroxidases themselves to be followed.
It is commonly used as a substrate with hydrogen peroxide for a peroxidase enzyme (such as horseradish peroxidase) or alone with blue multicopper oxidase enzymes (such as laccase or bilirubin oxidase). A common use for it is in the enzyme-linked immunosorbent assay ( ELISA) to detect the binding of molecules to each other. In biochemistry, ABTS ( 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) is a chemical compound used to observe the reaction kinetics of specific enzymes.
